Analysis of von Willebrand and Factor VIIIC Protein Using Partition Cells in the Analytical Ultracentrifuge
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چکیده
Sedimentation analysis of factor VIII complex was performed in the analytical ultracentrifuge using partition cells. This method allowed for the calculation of three different sedimentation coefficients from each run: one based on ristocetin agglutination activity for von Willebrand protein. 5WF; one based on coagulant activity for factor VIIIC. and one based on the schlieren or adsorption data for protein concentration. In most cases. there was no agreement between the three values calculated from the same run. indicating a heterogeneous system. The calculated functional sedimentation coefficients give values that require the molecules to be highly
منابع مشابه
Sedimentation analysis of von Willebrand and factor VIIIC protein using partition cells in the analytical ultracentrifuge.
Sedimentation analysis of factor VIII complex was performed in the analytical ultracentrifuge using partition cells. This method allowed for the calculation of three different sedimentation coefficients from each run: one based on ristocetin agglutination activity for von Willebrand protein, SWF; one based on coagulant activity for factor VIIIC, SVIIIC; and one based on the schlieren or adsorpt...
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